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- Title
A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion.
- Authors
Harterink, Martin; Port, Fillip; Lorenowicz, Magdalena J; McGough, Ian J; Silhankova, Marie; Betist, Marco C; van Weering, Jan R T; van Heesbeen, Roy G H P; Middelkoop, Teije C; Basler, Konrad; Cullen, Peter J; Korswagen, Hendrik C
- Abstract
Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding protein Wntless (Wls), which transports Wnt from the Golgi network to the cell surface for release. It has recently been shown that recycling of Wls through a retromer-dependent endosome-to-Golgi trafficking pathway is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is poorly understood. Here, we report that Wls recycling is mediated through a retromer pathway that is independent of the retromer sorting nexins SNX1-SNX2 and SNX5-SNX6. We have found that the unrelated sorting nexin, SNX3, has an evolutionarily conserved function in Wls recycling and Wnt secretion and show that SNX3 interacts directly with the cargo-selective subcomplex of the retromer to sort Wls into a morphologically distinct retrieval pathway. These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo.
- Publication
Nature cell biology, 2011, Vol 13, Issue 8, p914
- ISSN
1476-4679
- Publication type
Journal Article
- DOI
10.1038/ncb2281