We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Variegated gene expression caused by cell-specific long-range DNA interactions.
- Authors
Noordermeer, Daan; de Wit, Elzo; Klous, Petra; van de Werken, Harmen; Simonis, Marieke; Lopez-Jones, Melissa; Eussen, Bert; de Klein, Annelies; Singer, Robert H; de Laat, Wouter
- Abstract
Mammalian genomes contain numerous regulatory DNA sites with unknown target genes. We used mice with an extra β-globin locus control region (LCR) to investigate how a regulator searches the genome for target genes. We find that the LCR samples a restricted nuclear subvolume, wherein it preferentially contacts genes controlled by shared transcription factors. No contacted gene is detectably upregulated except for endogenous β-globin genes located on another chromosome. This demonstrates genetically that mammalian trans activation is possible, but suggests that it will be rare. Trans activation occurs not pan-cellularly, but in 'jackpot' cells enriched for the interchromosomal interaction. Therefore, cell-specific long-range DNA contacts can cause variegated expression.
- Publication
Nature cell biology, 2011, Vol 13, Issue 8, p944
- ISSN
1476-4679
- Publication type
Journal Article
- DOI
10.1038/ncb2278