We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Centriolar SAS-5 is required for centrosome duplication in C. elegans.
- Authors
Delattre, Marie; Leidel, Sebastian; Wani, Khursheed; Baumer, Karine; Bamat, Jeannine; Schnabel, Heinke; Feichtinger, Richard; Schnabel, Ralf; Gönczy, Pierre
- Abstract
Centrosomes, the major microtubule-organizing centres (MTOCs) of animal cells, are comprised of a pair of centrioles surrounded by pericentriolar material (PCM). Early in the cell cycle, there is a single centrosome, which duplicates during S-phase to direct bipolar spindle assembly during mitosis. Although crucial for proper cell division, the mechanisms that govern centrosome duplication are not fully understood. Here, we identify the Caenorhabditis elegans gene sas-5 as essential for daughter-centriole formation. SAS-5 is a coiled-coil protein that localizes primarily to centrioles. Fluorescence recovery after photobleaching (FRAP) experiments with green fluorescent protein (GFP) fused to SAS-5 (GFP-SAS-5) demonstrated that the protein shuttles between centrioles and the cytoplasm throughout the cell cycle. Analysis of mutant alleles revealed that the presence of SAS-5 at centrioles is crucial for daughter-centriole formation and that ZYG-1, a kinase that is also essential for this process, controls the distribution of SAS-5 to centrioles. Furthermore, partial RNA-interference (RNAi)-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication.
- Publication
Nature cell biology, 2004, Vol 6, Issue 7, p656
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/ncb1146