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- Title
Golgi targeting of ARF-like GTPase Arl3p requires its N<sup>a</sup>-acetylation and the integral membrane protein Sys1p.
- Authors
Setty, Subba Rao Gangi; Strochlic, Todd I.; Tong, Amy Hin Yan; Boone, Charles; Burd, Christopher G.
- Abstract
Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC Nα-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
- Publication
Nature Cell Biology, 2004, Vol 6, Issue 5, p414
- ISSN
1465-7392
- Publication type
Academic Journal
- DOI
10.1038/ncb1121