We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Loss of SM-B myosin affects muscle shortening velocity and maximal force development.
- Authors
Babu, G J; Loukianov, E; Loukianova, T; Pyne, G J; Huke, S; Osol, G; Low, R B; Paul, R J; Periasamy, M
- Abstract
We used an exon-specific gene-targeting strategy to generate a mouse model deficient only in the SM-B myosin isoform. Here we show that deletion of exon-5B (specific for SM-B) in the gene for the heavy chain of smooth muscle myosin results in a complete loss of SM-B myosin and switching of splicing to the SM-A isoform, without affecting SM1 and SM2 myosin content. Loss of SM-B myosin does not affect survival or cause any overt smooth muscle pathology. Physiological analysis reveals that absence of SM-B myosin results in a significant decrease in maximal force generation and velocity of shortening in smooth muscle tissues. This is the first in vivo study to demonstrate a functional role for the SM-B myosin isoform. We conclude that the extra seven-residue insert in the surface loop 1 of SM-B myosin is a critical determinant of crossbridge cycling and velocity of shortening.
- Publication
Nature cell biology, 2001, Vol 3, Issue 11, p1025
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/ncb1101-1025