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- Title
Selected lipids activate phagosome actin assembly and maturation resulting in killing of pathogenic mycobacteria.
- Authors
Anes, Elsa; Kühnel, Mark Philipp; Bos, Evelyne; Moniz-Pereira, Jose; Habermann, Anja; Griffiths, Gareth
- Abstract
Pathogenic mycobacteria such as Mycobacterium tuberculosis and Mycobacterium avium facilitate disease by surviving intracellularly within a potentially hostile environment: the macrophage phagosome. They inhibit phagosome maturation processes, including fusion with lysosomes, acidification and, as shown here, membrane actin assembly. An in vitro assay developed for latex bead phagosomes (LBPs) provided insights into membrane signalling events that regulate phagosome actin assembly, a process linked to membrane fusion. Different lipids were found to stimulate or inhibit actin assembly by LBPs and mycobacterial phagosomes in vitro. In addition, selected lipids activated actin assembly and phagosome maturation in infected macrophages, resulting in a significant killing of M. tuberculosis and M. avium. In contrast, the polyunsaturated sigma-3 lipids behaved differently and stimulated pathogen growth. Thus, lipids can be involved in both stimulatory and inhibitory signalling networks in the phagosomal membrane.
- Publication
Nature cell biology, 2003, Vol 5, Issue 9, p793
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/ncb1036