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- Title
Engineered riboregulators enable post-transcriptional control of gene expression.
- Authors
Isaacs, Farren J; Dwyer, Daniel J; Ding, Chunming; Pervouchine, Dmitri D; Cantor, Charles R; Collins, James J
- Abstract
Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the ribosome binding site in a target gene. Upon transcription, this cis-repressive sequence causes a stem-loop structure to form at the 5'-untranslated region of the mRNA. The stem-loop structure interferes with ribosome binding, silencing gene expression. A small noncoding RNA that is expressed in trans targets the cis-repressed RNA with high specificity, causing an alteration in the stem-loop structure that activates expression. Such engineered riboregulators may lend insight into mechanistic actions of endogenous RNA-based processes and could serve as scalable components of biological networks, able to function with any promoter or gene to directly control gene expression.
- Publication
Nature biotechnology, 2004, Vol 22, Issue 7, p841
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/nbt986