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- Title
Expression profiling reveals off-target gene regulation by RNAi.
- Authors
Jackson, Aimee L; Bartz, Steven R; Schelter, Janell; Kobayashi, Sumire V; Burchard, Julja; Mao, Mao; Li, Bin; Cavet, Guy; Linsley, Peter S
- Abstract
RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.
- Publication
Nature biotechnology, 2003, Vol 21, Issue 6, p635
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/nbt831