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- Title
Cloned zebrafish by nuclear transfer from long-term-cultured cells.
- Authors
Lee, Ki-Young; Huang, Haigen; Ju, Bensheng; Yang, Zhongan; Lin, Shuo
- Abstract
Although mammals have been cloned from genetically manipulated cultured cells, a comparable achievement has not been realized in lower vertebrates. Here we report that fertile transgenic zebrafish can be obtained by nuclear transfer using embryonic fibroblast cells from long-term cultures. The donor nuclei, modified by retroviral insertions expressing green fluorescent protein (GFP), were transplanted into manually enucleated eggs. Overall, a 2% success rate was achieved, resulting in 11 adult transgenic zebrafish expressing GFP. These nuclear transplants produced fertile, diploid offspring, and their F1/F2 progeny continued to express GFP in a pattern identical to that of the founder fish. This finding demonstrates that slowly dividing nuclei from cultured cells can be reprogrammed to support rapid embryonic development and sets up a foundation for targeted genetic manipulation in zebrafish.
- Publication
Nature biotechnology, 2002, Vol 20, Issue 8, p795
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/nbt721