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- Title
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
- Authors
Hyde, Stephen C; Pringle, Ian A; Abdullah, Syahril; Lawton, Anna E; Davies, Lee A; Varathalingam, Anusha; Nunez-Alonso, Graciela; Green, Anne-Marie; Bazzani, Reto P; Sumner-Jones, Stephanie G; Chan, Mario; Li, Hongyu; Yew, Nelson S; Cheng, Seng H; Boyd, A Christopher; Davies, Jane C; Griesenbach, Uta; Porteous, David J; Sheppard, David N; Munkonge, Felix M; Alton, Eric W F W; Gill, Deborah R
- Abstract
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.
- Publication
Nature biotechnology, 2008, Vol 26, Issue 5, p549
- ISSN
1546-1696
- Publication type
Journal Article
- DOI
10.1038/nbt1399