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- Title
Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol.
- Authors
Ying-Bo Mao; Wen-Juan Cai; Jia-Wei Wang; Gao-Jie Hong; Xiao-Yuan Tao; Ling-Jian Wang; Yong-Ping Huang; Xiao-Ya Chen
- Abstract
We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA–expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.
- Publication
Nature Biotechnology, 2007, Vol 25, Issue 11, p1307
- ISSN
1087-0156
- Publication type
Academic Journal
- DOI
10.1038/nbt1352