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- Title
Efficient genome editing in zebrafish using a CRISPR-Cas system.
- Authors
Hwang, Woong Y; Fu, Yanfang; Reyon, Deepak; Maeder, Morgan L; Tsai, Shengdar Q; Sander, Jeffry D; Peterson, Randall T; Yeh, J-R Joanna; Joung, J Keith
- Abstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
- Publication
Nature biotechnology, 2013, Vol 31, Issue 3, p227
- ISSN
1546-1696
- Publication type
Journal Article
- DOI
10.1038/nbt.2501