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- Title
Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.
- Authors
Warner, Joseph R; Reeder, Philippa J; Karimpour-Fard, Anis; Woodruff, Lauren B A; Gill, Ryan T
- Abstract
A fundamental goal in biotechnology and biology is the development of approaches to better understand the genetic basis of traits. Here we report a versatile method, trackable multiplex recombineering (TRMR), whereby thousands of specific genetic modifications are created and evaluated simultaneously. To demonstrate TRMR, in a single day we modified the expression of >95% of the genes in Escherichia coli by inserting synthetic DNA cassettes and molecular barcodes upstream of each gene. Barcode sequences and microarrays were then used to quantify population dynamics. Within a week we mapped thousands of genes that affect E. coli growth in various media (rich, minimal and cellulosic hydrolysate) and in the presence of several growth inhibitors (beta-glucoside, D-fucose, valine and methylglyoxal). This approach can be applied to a broad range of traits to identify targets for future genome-engineering endeavors.
- Publication
Nature biotechnology, 2010, Vol 28, Issue 8, p856
- ISSN
1546-1696
- Publication type
Journal Article
- DOI
10.1038/nbt.1653