We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A map of nucleosome positions in yeast at base-pair resolution.
- Authors
Brogaard, Kristin; Xi, Liqun; Wang, Ji-Ping; Widom, Jonathan
- Abstract
The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.
- Publication
Nature, 2012, Vol 486, Issue 7404, p496
- ISSN
1476-4687
- Publication type
Journal Article
- DOI
10.1038/nature11142