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- Title
Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B.
- Authors
Vivian, Julian P; Duncan, Renee C; Berry, Richard; O'Connor, Geraldine M; Reid, Hugh H; Beddoe, Travis; Gras, Stephanie; Saunders, Philippa M; Olshina, Maya A; Widjaja, Jacqueline M L; Harpur, Christopher M; Lin, Jie; Maloveste, Sebastien M; Price, David A; Lafont, Bernard A P; McVicar, Daniel W; Clements, Craig S; Brooks, Andrew G; Rossjohn, Jamie
- Abstract
Members of the killer cell immunoglobulin-like receptor (KIR) family, a large group of polymorphic receptors expressed on natural killer (NK) cells, recognize particular peptide-laden human leukocyte antigen (pHLA) class I molecules and have a pivotal role in innate immune responses. Allelic variation and extensive polymorphism within the three-domain KIR family (KIR3D, domains D0-D1-D2) affects pHLA binding specificity and is linked to the control of viral replication and the treatment outcome of certain haematological malignancies. Here we describe the structure of a human KIR3DL1 receptor bound to HLA-B*5701 complexed with a self-peptide. KIR3DL1 clamped around the carboxy-terminal end of the HLA-B*5701 antigen-binding cleft, resulting in two discontinuous footprints on the pHLA. First, the D0 domain, a distinguishing feature of the KIR3D family, extended towards β2-microglobulin and abutted a region of the HLA molecule with limited polymorphism, thereby acting as an 'innate HLA sensor' domain. Second, whereas the D2-HLA-B*5701 interface exhibited a high degree of complementarity, the D1-pHLA-B*5701 contacts were suboptimal and accommodated a degree of sequence variation both within the peptide and the polymorphic region of the HLA molecule. Although the two-domain KIR (KIR2D) and KIR3DL1 docked similarly onto HLA-C and HLA-B respectively, the corresponding D1-mediated interactions differed markedly, thereby providing insight into the specificity of KIR3DL1 for discrete HLA-A and HLA-B allotypes. Collectively, in association with extensive mutagenesis studies at the KIR3DL1-pHLA-B*5701 interface, we provide a framework for understanding the intricate interplay between peptide variability, KIR3D and HLA polymorphism in determining the specificity requirements of this essential innate interaction that is conserved across primate species.
- Publication
Nature, 2011, Vol 479, Issue 7373, p401
- ISSN
1476-4687
- Publication type
Journal Article
- DOI
10.1038/nature10517