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- Title
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
- Authors
Sato, Toshiro; Vries, Robert G; Snippert, Hugo J; van de Wetering, Marc; Barker, Nick; Stange, Daniel E; van Es, Johan H; Abo, Arie; Kujala, Pekka; Peters, Peter J; Clevers, Hans
- Abstract
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
- Publication
Nature, 2009, Vol 459, Issue 7244, p262
- ISSN
1476-4687
- Publication type
Journal Article
- DOI
10.1038/nature07935