We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Direct charging of tRNA<sub>CUA</sub> with pyrrolysine in vitro and in vivo.
- Authors
Blight, Sherry K.; Larue, Ross C.; Mahapatra, Anirban; Longstaff, David G.; Chang, Edward; Gang Zhao; Kang, Patrick T.; Green-Church, Kari B.; Chan, Michael K.; Krzycki, Joseph A.
- Abstract
Pyrrolysine is the 22nd amino acid. An unresolved question has been how this atypical genetically encoded residue is inserted into proteins, because all previously described naturally occurring aminoacyl-tRNA synthetases are specific for one of the 20 universally distributed amino acids. Here we establish that synthetic l-pyrrolysine is attached as a free molecule to tRNACUA by PylS, an archaeal class II aminoacyl-tRNA synthetase. PylS activates pyrrolysine with ATP and ligates pyrrolysine to tRNACUA in vitro in reactions specific for pyrrolysine. The addition of pyrrolysine to Escherichia coli cells expressing pylT (encoding tRNACUA) and pylS results in the translation of UAG in vivo as a sense codon. This is the first example from nature of direct aminoacylation of a tRNA with a non-canonical amino acid and shows that the genetic code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins.
- Publication
Nature, 2004, Vol 431, Issue 7006, p333
- ISSN
0028-0836
- Publication type
Academic Journal
- DOI
10.1038/nature02895