We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Targets of the cyclin-dependent kinase Cdk1.
- Authors
Ubersax, Jeffrey A; Woodbury, Erika L; Quang, Phuong N; Paraz, Maria; Blethrow, Justin D; Shah, Kavita; Shokat, Kevan M; Morgan, David O
- Abstract
The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have been identified. Here, we screened a budding yeast proteomic library for proteins that are directly phosphorylated by Cdk1 in whole-cell extracts. We identified about 200 Cdk1 substrates, several of which are phosphorylated in vivo in a Cdk1-dependent manner. The identities of these substrates reveal that Cdk1 employs a global regulatory strategy involving phosphorylation of other regulatory molecules as well as phosphorylation of the molecular machines that drive cell-cycle events. Detailed analysis of these substrates is likely to yield important insights into cell-cycle regulation.
- Publication
Nature, 2003, Vol 425, Issue 6960, p859
- ISSN
1476-4687
- Publication type
Journal Article
- DOI
10.1038/nature02062