We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Multiplex detection of hotspot mutations by rolling circle-enabled universal microarrays.
- Authors
Ladner, D P; Leamon, J H; Hamann, S; Tarafa, G; Strugnell, T; Dillon, D; Lizardi, P; Costa, J
- Abstract
Detection of somatic low abundance mutations in early cancer development requires a discriminatory, specific, and high-throughput methodology. In this study we report specific, discriminatory detection of low abundance mutations through a novel combination of rolling circle amplification (Nat Genet 1998; 19:225-232) and PCR ligation detection reaction on a universal oligonucleotide microarray (J Mol Biol 1999; 292:251-262). After mutation-specific multiplex ligation and hybridization of 17 pairs of probes to a generic microarray, the ligated probes were visualized. The multiplex mutation-specific ligation is possible only because rolling circle amplification permits quantification of previously undetectable hybridization events conducive to the detection of a single mutation from within a pool of over 100 wild-type alleles. This system is readily adaptable to high-throughput automation using a robot such as the Biomek platform.
- Publication
Laboratory investigation; a journal of technical methods and pathology, 2001, Vol 81, Issue 8, p1079
- ISSN
0023-6837
- Publication type
Journal Article
- DOI
10.1038/labinvest.3780320