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- Title
Anti-α-2-macroglobulin-like-1 autoantibodies are detected frequently and may be pathogenic in paraneoplastic pemphigus.
- Authors
Numata, Sanae; Teye, Kwesi; Tsuruta, Daisuke; Sogame, Ryosuke; Ishii, Norito; Koga, Hiroshi; Natsuaki, Yohei; Tsuchisaka, Atsunari; Hamada, Takahiro; Karashima, Tadashi; Nakama, Takekuni; Furumura, Minao; Ohata, Chika; Kawakami, Tamihiro; Schepens, Isabelle; Borradori, Luca; Hashimoto, Takashi
- Abstract
Paraneoplastic pemphigus (PNP) shows autoantibodies mainly to plakin and desmosomal cadherin family proteins. We have recently identified alpha-2-macroglobulin-like-1 (A2ML1), a broad range protease inhibitor, as a unique PNP antigen. In this study, we tested a large number of PNP sera by various methods. Forty (69.0%) of 58 PNP sera recognized A2ML1 recombinant protein expressed in COS7 cells by immunofluorescence (IF) and/or immunoprecipitation (IP)/immunoblotting (IB). IP/IB showed higher sensitivity than IF. In addition, 22 (37.9%) PNP sera reacted with A2ML1 by IB of cultured normal human keratinocytes (NHKs) under non-reducing conditions. Statistical analyses using various clinical and immunological data showed that the presence of anti-A2ML1 autoantibodies was associated with early disease onset and absence of ocular lesions. Next, to investigate the pathogenic role of anti-A2ML1 antibody, we performed additional functional studies. Addition of anti-A2ML1 polyclonal antibody to culture media decreased NHK cell adhesion examined by dissociation assay, and increased plasmin activity detected by casein zymography, suggesting that anti-A2ML1 antibody may decrease NHK cell adhesion through plasmin activation by inhibition of A2ML1. This study demonstrates that autoantibodies to A2ML1 are frequently and specifically detected and may have a pathogenic role in PNP.
- Publication
The Journal of investigative dermatology, 2013, Vol 133, Issue 7, p1785
- ISSN
1523-1747
- Publication type
Journal Article
- DOI
10.1038/jid.2013.65