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- Title
In vivo NADH fluorescence imaging indicates effect of aquaporin-4 deletion on oxygen microdistribution in cortical spreading depression.
- Authors
Thrane, Alexander S; Takano, Takahiro; Rangroo Thrane, Vinita; Wang, Fushun; Peng, Weiguo; Ottersen, Ole Petter; Nedergaard, Maiken; Nagelhus, Erlend A
- Abstract
Using in vivo two-photon imaging, we show that mice deficient in aquaporin-4 (AQP4) display increased fluorescence of nicotinamide adenine dinucleotide (NADH) when subjected to cortical spreading depression. The increased NADH signal, a proxy of tissue hypoxia, was restricted to microwatershed areas remote from the vasculature. Aqp4 deletion had no effects on the hyperemia response, but slowed [K(+)]o recovery. These observations suggest that K(+) uptake is suppressed in Aqp4(-/-) mice as a consequence of decreased oxygen delivery to tissue located furthest away from the vascular source of oxygen, although increased oxygen consumption may also contribute to our observations.
- Publication
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2013, Vol 33, Issue 7, p996
- ISSN
1559-7016
- Publication type
Journal Article
- DOI
10.1038/jcbfm.2013.63