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- Title
New method for isolating antibiotic-producing fungi.
- Authors
Kawaguchi, Mio; Nonaka, Kenichi; Masuma, Rokuro; Tomoda, Hiroshi
- Abstract
A convenient and efficient method was established for isolating antifungal antibiotic-producing fungi from soil samples. In this method, soil samples were diluted and directly plated in agar medium by the standard fungi-isolating method, and the plates were cultured at 27 °C for 2-3 days to permit the growth of fungal colonies. Then, the suspension of pathogenic Candida albicans in saline (40 μl, 5-10 × 10⁵ CFU ml⁻¹) was overlaid by spraying on the plates under controlled conditions in the safety cabinet. After 1-day incubation, fungal colonies showing an antagonistic effect with the inhibition zone against sprayed C. albicans were selected. Among 151 isolates, 26 strains were found to reproduce anti-C. albicans activity in liquid medium, yielding a higher selection rate (17.2%) than that (3.1%) by the traditional method. This new method can be applied for isolation of microorganisms (fungi and actinomycetes) that produce antibiotics active against pathogenic microorganisms.
- Publication
The Journal of antibiotics, 2013, Vol 66, Issue 1, p17
- ISSN
1881-1469
- Publication type
Journal Article
- DOI
10.1038/ja.2012.79