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- Title
CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing.
- Authors
Pirngruber, Judith; Shchebet, Andrei; Schreiber, Lisa; Shema, Efrat; Minsky, Neri; Chapman, Rob D; Eick, Dirk; Aylon, Yael; Oren, Moshe; Johnsen, Steven A
- Abstract
Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.
- Publication
EMBO reports, 2009, Vol 10, Issue 8, p894
- ISSN
1469-3178
- Publication type
Journal Article
- DOI
10.1038/embor.2009.108