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- Title
Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release.
- Authors
Malsam, Jörg; Parisotto, Daniel; Bharat, Tanmay A M; Scheutzow, Andrea; Krause, Jean Michel; Briggs, John A G; Söllner, Thomas H
- Abstract
Regulated exocytosis requires that the assembly of the basic membrane fusion machinery is temporarily arrested. Synchronized membrane fusion is then caused by a specific trigger--a local rise of the Ca(2+) concentration. Using reconstituted giant unilamellar vesicles (GUVs), we have analysed the role of complexin and membrane-anchored synaptotagmin 1 in arresting and synchronizing fusion by lipid-mixing and cryo-electron microscopy. We find that they mediate the formation and consumption of docked small unilamellar vesicles (SUVs) via the following sequence of events: Synaptotagmin 1 mediates v-SNARE-SUV docking to t-SNARE-GUVs in a Ca(2+)-independent manner. Complexin blocks vesicle consumption, causing accumulation of docked vesicles. Together with synaptotagmin 1, complexin synchronizes and stimulates rapid fusion of accumulated docked vesicles in response to physiological Ca(2+) concentrations. Thus, the reconstituted assay resolves both the stimulatory and inhibitory function of complexin and mimics key aspects of synaptic vesicle fusion.
- Publication
The EMBO journal, 2012, Vol 31, Issue 15, p3270
- ISSN
1460-2075
- Publication type
Journal Article
- DOI
10.1038/emboj.2012.164