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- Title
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
- Authors
El Yacoubi, Basma; Hatin, Isabelle; Deutsch, Christopher; Kahveci, Tamer; Rousset, Jean-Pierre; Iwata-Reuyl, Dirk; Murzin, Alexey G; de Crécy-Lagard, Valérie
- Abstract
The YgjD/Kae1 family (COG0533) has been on the top-10 list of universally conserved proteins of unknown function for over 5 years. It has been linked to DNA maintenance in bacteria and mitochondria and transcription regulation and telomere homeostasis in eukaryotes, but its actual function has never been found. Based on a comparative genomic and structural analysis, we predicted this family was involved in the biosynthesis of N(6)-threonylcarbamoyl adenosine, a universal modification found at position 37 of tRNAs decoding ANN codons. This was confirmed as a yeast mutant lacking Kae1 is devoid of t(6)A. t(6)A(-) strains were also used to reveal that t(6)A has a critical role in initiation codon restriction to AUG and in restricting frameshifting at tandem ANN codons. We also showed that YaeZ, a YgjD paralog, is required for YgjD function in vivo in bacteria. This work lays the foundation for understanding the pleiotropic role of this universal protein family.
- Publication
The EMBO journal, 2011, Vol 30, Issue 5, p882
- ISSN
1460-2075
- Publication type
Journal Article
- DOI
10.1038/emboj.2010.363