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- Title
Identification, structure, and functional requirement of the Mediator submodule Med7N/31.
- Authors
Koschubs, Tobias; Seizl, Martin; Larivière, Laurent; Kurth, Fabian; Baumli, Sonja; Martin, Dietmar E; Cramer, Patrick
- Abstract
Mediator is a modular multiprotein complex required for regulated transcription by RNA polymerase (Pol) II. Here, we show that the middle module of the Mediator core contains a submodule of unique structure and function that comprises the N-terminal part of subunit Med7 (Med7N) and the highly conserved subunit Med31 (Soh1). The Med7N/31 submodule shows a conserved novel fold, with two proline-rich stretches in Med7N wrapping around the right-handed four-helix bundle of Med31. In vitro, Med7N/31 is required for activated transcription and can act in trans when added exogenously. In vivo, Med7N/31 has a predominantly positive function on the expression of a specific subset of genes, including genes involved in methionine metabolism and iron transport. Comparative phenotyping and transcriptome profiling identify specific and overlapping functions of different Mediator submodules.
- Publication
The EMBO journal, 2009, Vol 28, Issue 1, p69
- ISSN
1460-2075
- Publication type
Journal Article
- DOI
10.1038/emboj.2008.254