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- Title
Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
- Authors
Zhang, Donghui; Jiang, Wei; Liu, Meng; Sui, Xin; Yin, Xiaolei; Chen, Song; Shi, Yan; Deng, Hongkui
- Abstract
Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells. Here we report a highly efficient approach to induce human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to differentiate into mature insulin-producing cells in a chemical-defined culture system. The differentiated human ES cells obtained by this approach comprised nearly 25% insulin-positive cells as assayed by flow cytometry analysis, which released insulin/C-peptide in response to glucose stimuli in a manner comparable to that of adult human islets. Most of these insulin-producing cells co-expressed mature β cell-specific markers such as NKX6-1 and PDX1, indicating a similar gene expression pattern to adult islet β cells in vivo. In this study, we also demonstrated that EGF facilitates the expansion of PDX1-positive pancreatic progenitors. Moreover, our protocol also succeeded in efficiently inducing human iPS cells to differentiate into insulin-producing cells. Therefore, this work not only provides a new model to study the mechanism of human pancreatic specialization and maturation in vitro, but also enhances the possibility of utilizing patient-specific iPS cells for the treatment of diabetes.Cell Research (2009) 19:429–438. doi: 10.1038/cr.2009.28; published online 3 March 2009
- Publication
Cell Research, 2009, Vol 19, Issue 4, p429
- ISSN
1001-0602
- Publication type
Academic Journal
- DOI
10.1038/cr.2009.28