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- Title
Auto-SCT induces a phenotypic shift from CMP to GMP progenitors, reduces clonogenic potential and enhances in vitro and in vivo cycling activity defined by <sup>18</sup>F-FLT PET scanning.
- Authors
Woolthuis, C.; Agool, A.; Olthof, S.; Slart, R. H J A; Huls, G.; Smid, W. M.; Schuringa, J. J.; Vellenga, E.
- Abstract
Autologous SCT (auto-SCT) introduces a reduced tolerance to chemotherapy even in patients with adequate engraftment, suggesting long-term effects of the transplantation procedure on the BM capacity. To study the hematopoietic cell compartment after auto-SCT, CD34+ BM cells (n=16) from patients at 6-9 months after auto-SCT were studied with regard to the progenitor subsets, colony frequency and cell cycle status. The BM compartments were studied in vivo using PET tracer 3-fluoro-3-deoxy-L-thymidine (18F-FLT PET). BM CD34+ cells after auto-SCT were compared with normal CD34+ cells and showed a phenotypic shift from common myeloid progenitor (CMP mean percentage 3.7 vs 19.4%, P=0.001) to granulocyte-macrophage progenitor (GMP mean percentage 51.8 vs 27.6%, P=0.01). In addition, a reduced clonogenic potential and higher cycling activity especially of the GMP fraction (41%±4 in G2/S phase vs 19%±2, P=0.03) were observed in BM after auto-SCT compared with normal. The enhanced cycling activity was confirmed in vivo by showing a significantly higher uptake of the 18F-FLT PET tracer by the BM compartment. This study shows that auto-SCT results in defects of the hematopoietic compartment at least 6 months after auto-SCT, characterized by changes in the composition of progenitor subsets and enhanced in vitro and in vivo cycling activity.
- Publication
Bone Marrow Transplantation, 2011, Vol 46, Issue 1, p110
- ISSN
0268-3369
- Publication type
Academic Journal
- DOI
10.1038/bmt.2010.75