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- Title
Functional analysis of secreted and transmembrane proteins critical to mouse development.
- Authors
Mitchell, K J; Pinson, K I; Kelly, O G; Brennan, J; Zupicich, J; Scherz, P; Leighton, P A; Goodrich, L V; Lu, X; Avery, B J; Tate, P; Dill, K; Pangilinan, E; Wakenight, P; Tessier-Lavigne, M; Skarnes, W C
- Abstract
We describe the successful application of a modified gene-trap approach, the secretory trap, to systematically analyze the functions in vivo of large numbers of genes encoding secreted and membrane proteins. Secretory-trap insertions in embryonic stem cells can be transmitted to the germ line of mice with high efficiency and effectively mutate the target gene. Of 60 insertions analyzed in mice, one-third cause recessive lethal phenotypes affecting various stages of embryonic and postnatal development. Thus, secretory-trap mutagenesis can be used for a genome-wide functional analysis of cell signaling pathways that are critical for normal mammalian development and physiology.
- Publication
Nature genetics, 2001, Vol 28, Issue 3, p241
- ISSN
1061-4036
- Publication type
Journal Article
- DOI
10.1038/90074