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- Title
Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1.
- Authors
Hopf, M; Göhring, W; Ries, A; Timpl, R; Hohenester, E
- Abstract
Nidogen, an invariant component of basement membranes, is a multifunctional protein that interacts with most other major basement membrane proteins. Here, we report the crystal structure of the mouse nidogen-1 G2 fragment, which contains binding sites for collagen IV and perlecan. The structure is composed of an EGF-like domain and an 11-stranded beta-barrel with a central helix. The beta-barrel domain has unexpected similarity to green fluorescent protein. A large surface patch on the beta-barrel is strikingly conserved in all metazoan nidogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding.
- Publication
Nature structural biology, 2001, Vol 8, Issue 7, p634
- ISSN
1072-8368
- Publication type
Journal Article
- DOI
10.1038/89683