We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Targeted screening for induced mutations.
- Authors
McCallum, C M; Comai, L; Greene, E A; Henikoff, S
- Abstract
With the accumulation of large-scale sequence data, emphasis in genomics has shifted from determining gene structure to testing gene function, and this relies on reverse genetic methodology. Here we explore the feasibility of screening for chemically induced mutations in target sequences in Arabidopsis thaliana. Our TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis. Importantly, this method generates a wide range of mutant alleles, is fast and automatable, and is applicable to any organism that can be chemically mutagenized.
- Publication
Nature biotechnology, 2000, Vol 18, Issue 4, p455
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/74542