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- Title
Analysis of vertebrate SCL loci identifies conserved enhancers.
- Authors
Göttgens, B; Barton, L M; Gilbert, J G; Bench, A J; Sanchez, M J; Bahn, S; Mistry, S; Grafham, D; McMurray, A; Vaudin, M; Amaya, E; Bentley, D R; Green, A R; Sinclair, A M
- Abstract
The SCL gene encodes a highly conserved bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis. We have sequenced and analyzed 320 kb of genomic DNA composing the SCL loci from human, mouse, and chicken. Long-range sequence comparisons demonstrated multiple peaks of human/mouse homology, a subset of which corresponded precisely with known SCL enhancers. Comparisons between mammalian and chicken sequences identified some, but not all, SCL enhancers. Moreover, one peak of human/mouse homology (+23 region), which did not correspond to a known enhancer, showed significant homology to an analogous region of the chicken SCL locus. A transgenic Xenopus reporter assay was established and demonstrated that the +23 region contained a new neural enhancer. This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers.
- Publication
Nature biotechnology, 2000, Vol 18, Issue 2, p181
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/72635