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- Title
Dynactin increases the processivity of the cytoplasmic dynein motor.
- Authors
King, S J; Schroer, T A
- Abstract
Cytoplasmic dynein supports long-range intracellular movements of cargo in vivo but does not appear to be a processive motor protein by itself. We show here that the dynein activator, dynactin, binds microtubules and increases the average length of cytoplasmic-dynein-driven movements without affecting the velocity or microtubule-stimulated ATPase kinetics of cytoplasmic dynein. Enhancement of microtubule binding and motility by dynactin are both inhibited by an antibody to dynactin's microtubule-binding domain. These results indicate that dynactin acts as a processivity factor for cytoplasmic-dynein-based motility and provide the first evidence that cytoskeletal motor processivity can be affected by extrinsic factors.
- Publication
Nature cell biology, 2000, Vol 2, Issue 1, p20
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/71338