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- Title
Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA.
- Authors
Antson, A A; Dodson, E J; Dodson, G; Greaves, R B; Chen, X; Gollnick, P
- Abstract
The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes of several bacilli by binding single-stranded RNA. The binding sequence is composed of eleven triplet repeats, predominantly GAG, separated by two or three non-conserved nucleotides. Here we present the crystal structure of a complex of TRAP and a 53-base single-stranded RNA containing eleven GAG triplets, revealing that each triplet is accommodated in a binding pocket formed by beta-strands. In the complex, the RNA has an extended structure without any base-pairing and binds to the protein mostly by specific protein-base interactions. Eleven binding pockets on the circular TRAP 11-mer form a belt with a diameter of about 80 A. This simple but elegant mechanism of arresting the RNA segment by encircling it around a protein disk is applicable to both transcription, when TRAP binds the nascent RNA, and to translation, when TRAP binds the same sequence within a non-coding leader region of the messenger RNA.
- Publication
Nature, 1999, Vol 401, Issue 6750, p235
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/45730