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- Title
The catalytic pathway of horseradish peroxidase at high resolution.
- Authors
Berglund, Gunnar I; Carlsson, Gunilla H; Smith, Andrew T; Szöke, Hanna; Henriksen, Anette; Hajdu, Janos
- Abstract
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
- Publication
Nature, 2002, Vol 417, Issue 6887, p463
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/417463a