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- Title
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
- Authors
Elbashir, S M; Harborth, J; Lendeckel, W; Yalcin, A; Weber, K; Tuschl, T
- Abstract
RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
- Publication
Nature, 2001, Vol 411, Issue 6836, p494
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/35078107