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- Title
Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling.
- Authors
Samatey, F A; Imada, K; Nagashima, S; Vonderviszt, F; Kumasaka, T; Yamamoto, M; Namba, K
- Abstract
The bacterial flagellar filament is a helical propeller constructed from 11 protofilaments of a single protein, flagellin. The filament switches between left- and right-handed supercoiled forms when bacteria switch their swimming mode between running and tumbling. Supercoiling is produced by two different packing interactions of flagellin called L and R. In switching from L to R, the intersubunit distance ( approximately 52 A) along the protofilament decreases by 0.8 A. Changes in the number of L and R protofilaments govern supercoiling of the filament. Here we report the 2.0 A resolution crystal structure of a Salmonella flagellin fragment of relative molecular mass 41,300. The crystal contains pairs of antiparallel straight protofilaments with the R-type repeat. By simulated extension of the protofilament model, we have identified possible switch regions responsible for the bi-stable mechanical switch that generates the 0.8 A difference in repeat distance.
- Publication
Nature, 2001, Vol 410, Issue 6826, p331
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/35066504