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- Title
Synaptotagmin I functions as a calcium regulator of release probability.
- Authors
Fernández-Chacón, R; Königstorfer, A; Gerber, S H; García, J; Matos, M F; Stevens, C F; Brose, N; Rizo, J; Rosenmund, C; Südhof, T C
- Abstract
In all synapses, Ca2+ triggers neurotransmitter release to initiate signal transmission. Ca2+ presumably acts by activating synaptic Ca2+ sensors, but the nature of these sensors--which are the gatekeepers to neurotransmission--remains unclear. One of the candidate Ca2+ sensors in release is the synaptic Ca2+-binding protein synaptotagmin I. Here we have studied a point mutation in synaptotagmin I that causes a twofold decrease in overall Ca2+ affinity without inducing structural or conformational changes. When introduced by homologous recombination into the endogenous synaptotagmin I gene in mice, this point mutation decreases the Ca2+ sensitivity of neurotransmitter release twofold, but does not alter spontaneous release or the size of the readily releasable pool of neurotransmitters. Therefore, Ca2+ binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
- Publication
Nature, 2001, Vol 410, Issue 6824, p41
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/35065004