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- Title
Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly.
- Authors
Carazo-Salas, R E; Gruss, O J; Mattaj, I W; Karsenti, E
- Abstract
It was recently reported that GTP-bound Ran induces microtubule and pseudo-spindle assembly in mitotic egg extracts in the absence of chromosomes and centrosomes, and that chromosomes induce the assembly of spindle microtubules in these extracts through generation of Ran-GTP. Here we examine the effects of Ran-GTP on microtubule nucleation and dynamics and show that Ran-GTP has independent effects on both the nucleation activity of centrosomes and the stability of centrosomal microtubules. We also show that inhibition of Ran-GTP production, even in the presence of duplicated centrosomes and kinetochores, prevents assembly of a bipolar spindle in M-phase extracts.
- Publication
Nature cell biology, 2001, Vol 3, Issue 3, p228
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/35060009