We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A transcription reinitiation intermediate that is stabilized by activator.
- Authors
Yudkovsky, N; Ranish, J A; Hahn, S
- Abstract
High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway. After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex. Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.
- Publication
Nature, 2000, Vol 408, Issue 6809, p225
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/35041603