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- Title
Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
- Authors
Yang, N; Higuchi, O; Ohashi, K; Nagata, K; Wada, A; Kangawa, K; Nishida, E; Mizuno, K
- Abstract
Rac is a small GTPase of the Rho family that mediates stimulus-induced actin cytoskeletal reorganization to generate lamellipodia. Little is known about the signalling pathways that link Rac activation to changes in actin filament dynamics. Cofilin is known to be a potent regulator of actin filament dynamics, and its ability to bind and depolymerize actin is abolished by phosphorylation of serine residue at 3; however, the kinases responsible for this phosphorylation have not been identified. Here we show that LIM-kinase 1 (LIMK-1), a serine/threonine kinase containing LIM and PDZ domains, phosphorylates cofilin at Ser 3, both in vitro and in vivo. When expressed in cultured cells, LIMK-1 induces actin reorganization and reverses cofilin-induced actin depolymerization. Expression of an inactive form of LIMK-1 suppresses lamellipodium formation induced by Rac or insulin. Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin.
- Publication
Nature, 1998, Vol 393, Issue 6687, p809
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/31735