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- Title
Crystal structure of a hepatitis delta virus ribozyme.
- Authors
Ferré-D'Amaré, A R; Zhou, K; Doudna, J A
- Abstract
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
- Publication
Nature, 1998, Vol 395, Issue 6702, p567
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/26912