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- Title
Retinoid-X receptor signalling in the developing spinal cord.
- Authors
Solomin, L; Johansson, C B; Zetterström, R H; Bissonnette, R P; Heyman, R A; Olson, L; Lendahl, U; Frisén, J; Perlmann, T
- Abstract
Retinoids regulate gene expression through the action of retinoic acid receptors (RARs) and retinoid-X receptors (RXRs), which both belong to the family of nuclear hormone receptors. Retinoids are of fundamental importance during development, but it has been difficult to assess the distribution of ligand-activated receptors in vivo. This is particularly the case for RXR, which is a critical unliganded auxiliary protein for several nuclear receptors, including RAR, but its ligand-activated role in vivo remains uncertain. Here we describe an assay in transgenic mice, based on the expression of an effector fusion protein linking the ligand-binding domain of either RXR or RAR to the yeast Gal4 DNA-binding domain, and the in situ detection of ligand-activated effector proteins by using an inducible transgenic lacZ reporter gene. We detect receptor activation in the spinal cord in a pattern that indicates that the receptor functions in the maturation of limb-innervating motor neurons. Our results reveal a specific activation pattern of Gal4-RXR which indicates that RXR is a critical bona fide receptor in the developing spinal cord.
- Publication
Nature, 1998, Vol 395, Issue 6700, p398
- ISSN
0028-0836
- Publication type
Journal Article
- DOI
10.1038/26515