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- Title
A microfabricated fluorescence-activated cell sorter.
- Authors
Fu, A Y; Spence, C; Scherer, A; Arnold, F H; Quake, S R
- Abstract
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting various biological entities. Compared with conventional FACS machines, the microFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used microFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
- Publication
Nature biotechnology, 1999, Vol 17, Issue 11, p1109
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/15095