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- Title
Rab5 regulates motility of early endosomes on microtubules.
- Authors
Nielsen, E; Severin, F; Backer, J M; Hyman, A A; Zerial, M
- Abstract
The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.
- Publication
Nature cell biology, 1999, Vol 1, Issue 6, p376
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/14075