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- Title
Formation of AP-3 transport intermediates requires Vps41 function.
- Authors
Rehling, P; Darsow, T; Katzmann, D J; Emr, S D
- Abstract
Transport of a subset of membrane proteins to the yeast vacuole requires the function of the AP-3 adaptor protein complex. To define the molecular requirements of vesicular transport in this pathway, we used a biochemical approach to analyse the formation and content of the AP-3 transport intermediate. A vam3tsf (vacuolar t-SNARE) mutant blocks vesicle docking and fusion with the vacuole and causes the accumulation of 50-130-nanometre membrane vesicles, which we isolated and showed by biochemical analysis and immunocytochemistry to contain both AP-3 adaptors and alkaline phosphatase (ALP) pathway cargoes. Inactivation of AP-3 or the protein Vps41 blocks formation of this vesicular intermediate. Vps41 binds to the AP-3 delta-adaptin subunit, suggesting that they function together in the formation of ALP pathway transport intermediates at the late Golgi.
- Publication
Nature cell biology, 1999, Vol 1, Issue 6, p346
- ISSN
1465-7392
- Publication type
Journal Article
- DOI
10.1038/14037