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- Title
A generic protein purification method for protein complex characterization and proteome exploration.
- Authors
Rigaut, G; Shevchenko, A; Rutz, B; Wilm, M; Mann, M; Séraphin, B
- Abstract
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
- Publication
Nature biotechnology, 1999, Vol 17, Issue 10, p1030
- ISSN
1087-0156
- Publication type
Journal Article
- DOI
10.1038/13732