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- Title
Sgf1p, a new component of the Sec34p/Sec35p complex.
- Authors
Kim, D W; Massey, T; Sacher, M; Pypaert, M; Ferro-Novick, S
- Abstract
Here we report the identification of SGF1 as a high-copy suppressor of the sec35-1 mutant. SGF1 encodes an essential hydrophilic protein of approximately 100 kDa. Using the yeast two-hybrid system and coprecipitation studies, we demonstrate that Sgf1p is a new subunit of the multiprotein Sec34p/Sec35p complex. Reduced levels of Sgf1p lead to the accumulation of a variety of membranes as well as a kinetic block in endoplasmic reticulum to Golgi traffic. Immunofluorescence studies demonstrate that Sec34p is found throughout the Golgi, with a high concentration on early Golgi. Although an earlier study suggested that Sec34p (Grd20p) is not required for protein secretion, we show here that the sec34-2 and sec35-1 mutations lead to a pleiotropic block in the secretion of all proteins into the growth medium.
- Publication
Traffic (Copenhagen, Denmark), 2001, Vol 2, Issue 11, p820
- ISSN
1398-9219
- Publication type
Journal Article
- DOI
10.1034/j.1600-0854.2001.21111.x