We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Total internal reflection fluorescence microscopy in cell biology.
- Authors
Axelrod, D
- Abstract
Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell-substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope.
- Publication
Traffic (Copenhagen, Denmark), 2001, Vol 2, Issue 11, p764
- ISSN
1398-9219
- Publication type
Journal Article
- DOI
10.1034/j.1600-0854.2001.21104.x