We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
CDw108 expression during T-cell development.
- Authors
Mine, T; Harada, K; Matsumoto, T; Yamana, H; Shirouzu, K; Itoh, K; Yamada, A
- Abstract
We recently reported a gene encoding the human CDw108, a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is preferentially expressed on activated T lymphocytes and erythrocytes. The present study investigated the expression of CDw108 on various tissues and cells, particularly on T cells during development. The murine CDw108 cDNA was cloned initially, and it was highly homologous to the human CDw108 (88.0% or 89.3% similarity at the nucleotide or amino acid level, respectively) or identical to the murine semaphorin K1/Sema7A. The CDw108 mRNA was demonstrated in a few tissues including thymus and brain with the highest expression coming on day 7 in whole embryo followed by relatively consistent expression during development. Cell-surface expression of the CDw108 during T-cell development was further examined by flow cytometry in the human umbilical cord blood and thymus. It was preferentially expressed on a CD34+ stem cell population of umbilical cord blood, and CD3dull CD34+/- CD117 (c-kit)+ CD4bright CDbright cells in the thymus that are involved in the stage of positive selection. These results suggest the contribution of CDw108 in T-cell development, especially in the stage of positive selection in the thymus.
- Publication
Tissue antigens, 2000, Vol 55, Issue 5, p429
- ISSN
0001-2815
- Publication type
Journal Article
- DOI
10.1034/j.1399-0039.2000.550505.x